磷酸二酯酶I

英文名稱：Phosphodiesterase I from Crotalus adamanteus venom；5′-Exonuclease；Oligonucleate 5′-nucleotidohydrolase

其他名稱：磷酸二脂酶；磷酸二酯酶

CAS號：9025-82-5

級別：BR

Specific activity：≥20u/mg protein

活力定義：One Unit hydrolyzes one micromole of p-nitrophenylthymidine-5-phosphate per minute at 25℃, pH 8.9.

產(chǎn)品描述：Venom exonuclease (Phosphodiesterase I) successivelyhydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- anddeoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 anda molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducingagents such as glutathione, cysteine and ascorbic acids. It is completelyinhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzymehas an absolute requirement for Mg2+

性狀(以下信息僅供參考)：白色或類白色粉末

用途：本品僅供科研，不得用于其它用途。(以下用途僅供參考)用于序列核酸的研究

保存：-20℃